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Isolation of Nuclei for use in Genome-wide DNase Hypersensitivity Assays to Probe Chromatin Structure
DNase hypersensitivity (DHS) analysis coupled with high throughput DNA sequencing (DNase-seq) has emerged as a powerful tool to analyze chromatin accessibility and identify regulatory sequences in genomic DNA on a global scale. In this method, intact nuclei are isolated from fresh tissue or cultured...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3815455/ https://ncbi.nlm.nih.gov/pubmed/23436350 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-284-1_2 |
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