Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins

Although Pichia pastoris is a popular protein expression system, it exhibits limitations in its ability to secrete heterologous proteins. Therefore, a REMI (restriction enzyme mediated insertion) strategy was utilized to select mutant beta-galactosidase supersecretion (bgs) strains that secreted inc...

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Autors principals: Larsen, Sasha, Weaver, Jun, de Sa Campos, Katherine, Bulahan, Rhobe, Nguyen, Jackson, Grove, Heather, Huang, Amy, Low, Lauren, Tran, Namphuong, Gomez, Seth, Yau, Jennifer, Ilustrisimo, Thomas, Kawilarang, Jessica, Lau, Jonathan, Tranphung, Maivi, Chen, Irene, Tran, Christina, Fox, Marcia, Lin-Cereghino, Joan, Lin-Cereghino, Geoff P.
Format: Artigo
Idioma:Inglês
Publicat: 2013
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3814129/
https://ncbi.nlm.nih.gov/pubmed/23881328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-013-1290-7
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