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Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins
Although Pichia pastoris is a popular protein expression system, it exhibits limitations in its ability to secrete heterologous proteins. Therefore, a REMI (restriction enzyme mediated insertion) strategy was utilized to select mutant beta-galactosidase supersecretion (bgs) strains that secreted inc...
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| 主要な著者: | , , , , , , , , , , , , , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2013
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3814129/ https://ncbi.nlm.nih.gov/pubmed/23881328 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-013-1290-7 |
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