The Effect of α-Mating Factor Secretion Signal Mutations on Recombinant Protein Expression in Pichia pastoris

The methylotrophic yeast, Pichia pastoris, has been genetically engineered to produce many heterologous proteins for industrial and research purposes. In order to secrete proteins for easier purification from the extracellular medium, the coding sequence of recombinant proteins are initially fused t...

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Main Authors: Lin-Cereghino, Geoff P., Stark, Carolyn M., Kim, Daniel, Chang, Jennifer, Shaheen, Nadia, Poerwanto, Hansel, Agari, Kimiko, Moua, Pachai, Low, Lauren K., Tran, Namphuong, Huang, Amy D., Nattestad, Maria, Oshiro, Kristin T., Chang, John William, Chavan, Archana, Tsai, Jerry W., Lin-Cereghino, Joan
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3628533/
https://ncbi.nlm.nih.gov/pubmed/23454485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gene.2013.01.062
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