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Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Genetics Society of America
2013
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3813845/ https://ncbi.nlm.nih.gov/pubmed/23979577 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.155879 |
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