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Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3813845/ https://ncbi.nlm.nih.gov/pubmed/23979577 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.155879 |
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