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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at speci...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3813849/ https://ncbi.nlm.nih.gov/pubmed/23979586 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.156299 |
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