CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at speci...

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Bibliografiset tiedot
Päätekijät: Waaijers, Selma, Portegijs, Vincent, Kerver, Jana, Lemmens, Bennie B. L. G., Tijsterman, Marcel, van den Heuvel, Sander, Boxem, Mike
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Genetics Society of America 2013
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Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3813849/
https://ncbi.nlm.nih.gov/pubmed/23979586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.156299
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