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Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans
Improved genome engineering methods that enable automation of large and precise edits are essential for systematic investigations of genome function. We adapted peel-1 negative selection to an optimized Dual-Marker Selection (DMS) cassette protocol for CRISPR-Cas9 genome engineering in Caenorhabditi...
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| Veröffentlicht in: | PLoS One |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7508457/ https://ncbi.nlm.nih.gov/pubmed/32960926 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0238950 |
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