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Transmembrane helix heterodimerization in lipid bilayers: probing the energetics behind autosomal dominant growth disorders
Here we show that the energetics of TM helix heterodimer formation can be characterized in liposomes using FRET. We present the theory and the protocol for measuring the free energy of heterodimerization, and the total (hetero and homo-dimeric) dimer fraction. We use the presented methodology to det...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2006
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3812923/ https://ncbi.nlm.nih.gov/pubmed/16500676 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2006.01.086 |
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