Saccharomyces cerevisiae–Based Platform for Rapid Production and Evaluation of Eukaryotic Nutrient Transporters and Transceptors for Biochemical Studies and Crystallography

To produce large quantities of high quality eukaryotic membrane proteins in Saccharomyces cerevisiae, we modified a high-copy vector to express membrane proteins C-terminally-fused to a Tobacco Etch Virus (TEV) protease detachable Green Fluorescent Protein (GFP)-8His tag, which facilitates localizat...

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Bibliografiske detaljer
Main Authors: Scharff-Poulsen, Peter, Pedersen, Per Amstrup
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3790737/
https://ncbi.nlm.nih.gov/pubmed/24124599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0076851
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