Improved Detection of Rare HIV-1 Variants using 454 Pyrosequencing

454 pyrosequencing, a massively parallel sequencing (MPS) technology, is often used to study HIV genetic variation. However, the substantial mismatch error rate of the PCR required to prepare HIV-containing samples for pyrosequencing has limited the detection of rare variants within viral population...

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Autors principals: Larsen, Brendan B., Chen, Lennie, Maust, Brandon S., Kim, Moon, Zhao, Hong, Deng, Wenjie, Westfall, Dylan, Beck, Ingrid, Frenkel, Lisa M., Mullins, James I.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2013
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3788733/
https://ncbi.nlm.nih.gov/pubmed/24098517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0076502
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