Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast

We have constructed IgG1-Fc scaffolds with increased thermal stability by directed evolution and yeast surface display. As a basis a new selection strategy that allowed the application of yeast surface display for screening of stabilizing mutations in proteins of already high intrinsic thermal stabi...

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Autores principales: Traxlmayr, Michael W., Faissner, Maximilian, Stadlmayr, Gerhard, Hasenhindl, Christoph, Antes, Bernhard, Rüker, Florian, Obinger, Christian
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier Pub. Co 2012
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3787792/
https://ncbi.nlm.nih.gov/pubmed/22285845
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2012.01.006
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