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Resin-assisted Enrichment of N-terminal Peptides for Characterizing Proteolytic Processing

A resin-assisted enrichment method has been developed for specific isolation of protein N-terminal peptides to facilitate LC-MS/MS characterization of proteolytic processing, a major form of posttranslational modifications. In this method, protein thiols are blocked by reduction and alkylation, and...

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Dettagli Bibliografici
Autori principali: Kim, Jong-Seo, Dai, Ziyu, Aryal, Uma K., Moore, Ronald J., Camp, David G., Baker, Scott E., Smith, Richard D., Qian, Wei-Jun
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3782086/
https://ncbi.nlm.nih.gov/pubmed/23772796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac401000q
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