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Resin-assisted Enrichment of N-terminal Peptides for Characterizing Proteolytic Processing
A resin-assisted enrichment method has been developed for specific isolation of protein N-terminal peptides to facilitate LC-MS/MS characterization of proteolytic processing, a major form of posttranslational modifications. In this method, protein thiols are blocked by reduction and alkylation, and...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3782086/ https://ncbi.nlm.nih.gov/pubmed/23772796 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac401000q |
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