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Tb(3+)-tRNA for LRET studies of Protein Synthesis

When suitably labeled bulk tRNAs are transfected into cells they give rise to FRET (fluorescence resonance energy transfer) signals via binding to ribosomes that provide a measure of total protein synthesis. Application of this approach to monitoring rates of specific protein synthesis requires achi...

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Detalhes bibliográficos
Main Authors: Alonso, Dulce, Liu, Wei, Rosenblum, Gabriel, Mani, Tomoyasu, Goldman, Yale E., Cooperman, Barry S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3779508/
https://ncbi.nlm.nih.gov/pubmed/23734598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc400062d
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