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Real-time assay for testing components of protein synthesis
We present a flexible, real-time-coupled transcription–translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along with numerical simulation of a reaction kinetics model, the assay permits quantitative estimation of the effects on full-length protein synthes...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3384345/ https://ncbi.nlm.nih.gov/pubmed/22422844 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks232 |
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