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The pH dependence of staphylococcal nuclease stability is incompatible with a three-state denaturation model
Six single substitution mutations, V66F, V66G, V66N, V66Q, V66S, V66T, and V66Y, were made in the background of a highly stable triple mutant (P117G, H124L, and S128A) of staphylococcal nuclease. The thermodynamic stabilities of wild type staphylococcal nuclease, of the stable triple mutant and of i...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2013
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3770747/ https://ncbi.nlm.nih.gov/pubmed/23892194 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2013.06.018 |
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