Cold denaturation and 2H2O stabilization of a staphylococcal nuclease mutant.

Cold denaturation is now recognized as a general property of proteins but has been observed only under destabilizing conditions, such as moderate denaturant concentration or low pH. By destabilizing the protein using site-directed mutagenesis, we have observed cold denaturation at pH 7.0 in the abse...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Antonino, L C, Kautz, R A, Nakano, T, Fox, R O, Fink, A L
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52373/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1652762/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.17.7715
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