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Thermal Denaturations of Staphylococcal Nuclease Wild-Type and Mutants Monitored by Fluorescence and Circular Dichroism are Similar: Lack of Evidence for Other Than a Two State Thermal Denaturation
It is unclear whether the thermal denaturation of staphylococcal nuclease is a two state, three state, or variable two state process. The thermal denaturation of wild-type staphylococcal nuclease was followed by tryptophan fluorescence and circular dichroism signal at 222 nm, forty-two and fourteen...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2006
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1941688/ https://ncbi.nlm.nih.gov/pubmed/17134819 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2006.10.014 |
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