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Photocaged T7 RNA Polymerase for the Light Activation of Transcription and Gene Function in Pro- and Eukaryotic Cells

A light-activatable bacteriophage T7 RNA polymerase (T7RNAP) has been generated through the site-specific introduction of a photocaged tyrosine residue at the crucial position Tyr639 within the active site of the enzyme. The photocaged tyrosine disrupts polymerase activity by blocking the incoming n...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chou, Chungjung, Young, Douglas D., Deiters, Alexander
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3762680/
https://ncbi.nlm.nih.gov/pubmed/20301166
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201000041
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