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Light activation of transcription: photocaging of nucleotides for control over RNA polymerization
We describe the use of ATP caged with [7-(diethylamino)coumarin-4-yl]methyl (DEACM) for light-controlled in vitro transcription reactions. Polymerization is blocked when DEACM is bonded to the gamma phosphate group of the ATP molecule. Controlled light irradiation releases ATP and transcription is i...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2504310/ https://ncbi.nlm.nih.gov/pubmed/18586819 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn415 |
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