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Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila
We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disrupt...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3761309/ https://ncbi.nlm.nih.gov/pubmed/23833182 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.153825 |
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