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A Microfluidic Approach for Protein Structure Determination at Room Temperature via on-chip Anomalous Diffraction

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11Å via on-chip collection of anomalous data tha...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Perry, Sarah L., Guha, Sudipto, Pawate, Ashtamurthy S., Bhaskarla, Amrit, Agarwal, Vinayak, Nair, Satish K., Kenis, Paul J.A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2013
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3755953/
https://ncbi.nlm.nih.gov/pubmed/23828485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3lc50276g
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