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Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells
Many biological processes rely on membrane fusion, therefore assays to study its mechanisms are necessary. Here we report an assay with sensitivity to single-vesicle, even to single-molecule events using fluorescently labeled vesicle-associated v-SNARE liposomes and target-membrane-associated t-SNAR...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3747733/ https://ncbi.nlm.nih.gov/pubmed/22517259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.019 |
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