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Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point
We demonstrate the use of a microfluidic stagnation point flow to trap and extend single molecules of double-stranded (ds) genomic DNA for detection of target sequences along the DNA backbone. Mutant EcoRI-based fluorescent markers are bound sequence-specifically to fluorescently labeled ds λ-DNA. T...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3322643/ https://ncbi.nlm.nih.gov/pubmed/20358051 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/b926847b |
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