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Fluorescent marker for direct detection of specific dsDNA sequences
We have created a fluorescent marker using a mutant EcoRI restriction endonuclease (K249C) that enables prolonged, direct visualization of specific sequences on genomic lengths of double-stranded (ds) DNA. The marker consists of a biotinylated enzyme, attached through the biotin-avidin interaction t...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2009
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2811260/ https://ncbi.nlm.nih.gov/pubmed/19908852 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac9019895 |
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