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Fluorescent marker for direct detection of specific dsDNA sequences

We have created a fluorescent marker using a mutant EcoRI restriction endonuclease (K249C) that enables prolonged, direct visualization of specific sequences on genomic lengths of double-stranded (ds) DNA. The marker consists of a biotinylated enzyme, attached through the biotin-avidin interaction t...

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Bibliografske podrobnosti
Main Authors: Dylla-Spears, Rebecca, Townsend, Jacqueline E., Sohn, Lydia L., Jen-Jacobson, Linda, Muller, Susan J.
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2811260/
https://ncbi.nlm.nih.gov/pubmed/19908852
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac9019895
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