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Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells

Many biological processes rely on membrane fusion, therefore assays to study its mechanisms are necessary. Here we report an assay with sensitivity to single-vesicle, even to single-molecule events using fluorescently labeled vesicle-associated v-SNARE liposomes and target-membrane-associated t-SNAR...

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Detalhes bibliográficos
Main Authors: Karatekin, Erdem, Rothman, James E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3747733/
https://ncbi.nlm.nih.gov/pubmed/22517259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.019
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