Determination of Neuraminidase Kinetic Constants Using Whole Influenza Virus Preparations and Correction for Spectroscopic Interference by a Fluorogenic Substrate

The influenza neuraminidase (NA) enzyme cleaves terminal sialic acid residues from cellular receptors, a process required for the release of newly synthesized virions. A balance of NA activity with sialic acid binding affinity of hemagglutinin (HA) is important for optimal virus replication. NA sequ...

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Main Authors: Marathe, Bindumadhav M., Lévêque, Vincent, Klumpp, Klaus, Webster, Robert G., Govorkova, Elena A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3744557/
https://ncbi.nlm.nih.gov/pubmed/23977037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071401
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