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Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses
Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process....
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373311/ https://ncbi.nlm.nih.gov/pubmed/14704360 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh009 |
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