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Improved generation of recombinant baculovirus genomes in Escherichia coli
An improved method for the generation of recombinant baculoviruses by Tn7-mediated transposition is described. The method is based on the modified donor vector (pBVboost) and an improved selection scheme of the baculovirus bacmids in Escherichia coli with a mutated SacB gene. Recombinant bacmids can...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2003
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC212821/ https://ncbi.nlm.nih.gov/pubmed/12930975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng102 |
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