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pH-dependent association of factor VIII chains: Enhancement of affinity at physiological pH by Cu(2+)
Reconstitution of factor VIII from isolated heavy chain (HC) and light chain (LC) shows pH-dependence. In the presence of Ca(2+), up to 80% of native factor VIII activity was recovered over a wide range of pH. In contrast, affinity of HC and LC was maximal at pH 6.5–6.75 (K(d) ~ 4 nM), whereas a K(d...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3731048/ https://ncbi.nlm.nih.gov/pubmed/16731058 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2006.04.004 |
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