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Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002
Alanine dehydrogenase (l-AlaDH) from Bacillus megaterium WSH-002 catalyses the NAD(+)-dependent interconversion of l-alanine and pyruvate. The enzyme was expressed in Escherichia coli BL21 (DE3) cells and purified with a His(6) tag by Ni(2+)-chelating affinity chromatography for X-ray crystallograp...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3729178/ https://ncbi.nlm.nih.gov/pubmed/23908047 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113019672 |
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