Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002

Alanine dehydrogenase (l-AlaDH) from Bacillus megaterium WSH-002 catalyses the NAD(+)-dependent interconversion of l-alanine and pyruvate. The enzyme was expressed in Escherichia coli BL21 (DE3) cells and purified with a His(6) tag by Ni(2+)-chelating affinity chromatography for X-ray crystallo­grap...

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Hauptverfasser: Lu, Xiaoyun, Yi, Qiufen, Zhang, Guofang, Zhu, Xianming, Zhou, Honggang, Dong, Hui
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2013
Schlagworte:
Crystallization Communications
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3729178/
https://ncbi.nlm.nih.gov/pubmed/23908047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113019672
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