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Crystallization and preliminary X-ray study of a (2R,3R)-2,3-butanediol dehydrogenase from Bacillus coagulans 2-6

(2R,3R)-2,3-Butanediol dehydrogenase (R,R-BDH) from Bacillus coagulans 2-6 is a zinc-dependent medium-chain alcohol dehydrogenase. Recombinant R,R-BDH with a His(6) tag at the C-terminus was expressed in Escherichia coli BL21 (DE3) cells and purified by Ni(2+)-chelating affinity and size-exclusion c...

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Detalhes bibliográficos
Main Authors: Miao, Xiangzhi, Huang, Xianhui, Zhang, Guofang, Zhao, Xiufang, Zhu, Xianming, Dong, Hui
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792675/
https://ncbi.nlm.nih.gov/pubmed/24100567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911302410X
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