Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila

Kluyvera citrophila penicillin G acylase (KcPGA) has recently attracted increased attention relative to the well studied and commonly used Escherichia coli PGA (EcPGA) because KcPGA is more resilient to harsh conditions and is easier to immobilize for the industrial hydrolysis of natural penicillins...

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Detalhes bibliográficos
Main Authors: Varshney, Nishant Kumar, Ramasamy, Sureshkumar, Brannigan, James A., Wilkinson, Anthony J., Suresh, C. G.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3729176/
https://ncbi.nlm.nih.gov/pubmed/23908045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911301943X
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