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Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis
Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their β-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity....
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| Auteurs principaux: | , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
International Union of Crystallography
2005
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1952454/ https://ncbi.nlm.nih.gov/pubmed/16511127 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105017987 |
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