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Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants

The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzy...

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Detalhes bibliográficos
Main Authors: Chandra, P. Manish, Brannigan, James A., Prabhune, Asmita, Pundle, Archana, Turkenburg, Johan P., Dodson, G. Guy, Suresh, C. G.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952408/
https://ncbi.nlm.nih.gov/pubmed/16508111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104031227
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