Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion

The fidelity of DNA polymerases depends on conformational changes that promote the rejection of incorrect nucleotides before phosphoryl transfer. Here, we combine single-molecule FRET with the use of DNA polymerase I and various fidelity mutants to highlight mechanisms by which active-site side chai...

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Hlavní autoři: Hohlbein, Johannes, Aigrain, Louise, Craggs, Timothy D., Bermek, Oya, Potapova, Olga, Shoolizadeh, Pouya, Grindley, Nigel D. F., Joyce, Catherine M., Kapanidis, Achillefs N.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Pub. Group 2013
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3715850/
https://ncbi.nlm.nih.gov/pubmed/23831915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms3131
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