Simultaneous Surface Display and Secretion of Proteins from Mammalian Cells Facilitate Efficient in Vitro Selection and Maturation of Antibodies

A mammalian expression system has been developed that permits simultaneous cell surface display and secretion of the same protein through alternate splicing of pre-mRNA. This enables a flexible system for in vitro protein evolution in mammalian cells where the displayed protein phenotype remains lin...

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Détails bibliographiques
Auteurs principaux: Horlick, Robert A., Macomber, John L., Bowers, Peter M., Neben, Tamlyn Y., Tomlinson, Geoffery L., Krapf, Irina P., Dalton, Jennifer L., Verdino, Petra, King, David J.
Format: Artigo
Langue:Inglês
Publié: American Society for Biochemistry and Molecular Biology 2013
Sujets:
Immunology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3707688/
https://ncbi.nlm.nih.gov/pubmed/23689374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.452482
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