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Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence
We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protei...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3701896/ https://ncbi.nlm.nih.gov/pubmed/23827883 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep02151 |
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