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Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence

We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protei...

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Bibliografiske detaljer
Main Authors: Oikawa, Hiroyuki, Suzuki, Yuta, Saito, Masataka, Kamagata, Kiyoto, Arai, Munehito, Takahashi, Satoshi
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3701896/
https://ncbi.nlm.nih.gov/pubmed/23827883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep02151
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