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An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity

We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aaRSs against an array of unnatural amino acids. We discovered that a p-cyanophenylalanine specific aminoacyl-tRNA synthetase (pCNF-RS) has high substrate permissivity for unnatural amino acids, while maintai...

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Bibliografische gegevens
Hoofdauteurs: Young, Douglas D., Young, Travis S., Jahnz, Michael, Ahmad, Insha, Spraggon, Glen, Schultz, Peter G.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2011
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3694404/
https://ncbi.nlm.nih.gov/pubmed/21280675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101929e
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