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An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity
We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aaRSs against an array of unnatural amino acids. We discovered that a p-cyanophenylalanine specific aminoacyl-tRNA synthetase (pCNF-RS) has high substrate permissivity for unnatural amino acids, while maintai...
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| Autores principales: | , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3694404/ https://ncbi.nlm.nih.gov/pubmed/21280675 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101929e |
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