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In vivo imaging and biochemical characterization of protease function using fluorescent activity-based probes

Activity-based probes (ABPs) are reactive small molecules that covalently bind to active enzymes. When tagged with a fluorophore, ABPs serve as powerful tools to investigate enzymatic activity across a wide variety of applications. In this article, we will provide detailed protocols for using fluore...

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Detalhes bibliográficos
Main Authors: Edgington, Laura E., Bogyo, Matthew
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3691694/
https://ncbi.nlm.nih.gov/pubmed/23788323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch120235
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