Pichia pastoris as a host system for transformations.
We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors w...
Gespeichert in:
| Veröffentlicht in: | Mol Cell Biol |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Taylor & Francis
1985
|
| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369166/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3915774/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.12.3376-3385.1985 |
| Tags: |
Keine Tags, Fügen Sie das erste Tag hinzu!
|