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Pichia pastoris as a host system for transformations.

We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors w...

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Sonraí Bibleagrafaíochta
Main Authors: Cregg, J M, Barringer, K J, Hessler, A Y, Madden, K R
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1985
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC369166/
https://ncbi.nlm.nih.gov/pubmed/3915774
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