Pichia pastoris as a host system for transformations.
We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors w...
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| Опубліковано в:: | Mol Cell Biol |
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| Автори: | , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Taylor & Francis
1985
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369166/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3915774/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.12.3376-3385.1985 |
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