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Pichia pastoris as a host system for transformations.

We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors w...

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Bibliografiska uppgifter
I publikationen:Mol Cell Biol
Huvudupphov: Cregg, J M, Barringer, K J, Hessler, A Y, Madden, K R
Materialtyp: Artigo
Språk:Inglês
Utgiven: Taylor & Francis 1985
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369166/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3915774/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.12.3376-3385.1985
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