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Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions

[Image: see text] Rapid, site-specific labeling of proteins with diverse probes remains an outstanding challenge for chemical biologists. Enzyme-mediated labeling approaches may be rapid but use protein or peptide fusions that introduce perturbations into the protein under study and may limit the si...

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Autori principali: Lang, Kathrin, Davis, Lloyd, Wallace, Stephen, Mahesh, Mohan, Cox, Daniel J., Blackman, Melissa L., Fox, Joseph M., Chin, Jason W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2012
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3687367/
https://ncbi.nlm.nih.gov/pubmed/22694658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja302832g
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