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Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions
[Image: see text] Rapid, site-specific labeling of proteins with diverse probes remains an outstanding challenge for chemical biologists. Enzyme-mediated labeling approaches may be rapid but use protein or peptide fusions that introduce perturbations into the protein under study and may limit the si...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical Society
2012
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3687367/ https://ncbi.nlm.nih.gov/pubmed/22694658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja302832g |
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