Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli

BACKGROUND: Activators of Nitrile hydratase (NHase) are essential for functional NHase biosynthesis. However, the activator P14K in P. putida is difficult to heterogeneously express, which retards the clarification of the mechanism of P14K involved in the maturation of NHase. Although a strep tag co...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Liu, Yi, Cui, Wenjing, Fang, Yueqin, Yu, Yuechun, Cui, Youtian, Xia, Yuanyuan, Kobayashi, Michihiko, Zhou, Zhemin
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2013
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3680314/
https://ncbi.nlm.nih.gov/pubmed/23731949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-48
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