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Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115
BACKGROUND: The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from Pseudomonas putida, various strategies for the over-expression of P14K are n...
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| Publicado no: | BMC Biotechnol |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4761151/ https://ncbi.nlm.nih.gov/pubmed/26897378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0252-2 |
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